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This system involved the administration of anabolic steroids on rats, either orally or by injection (depending on the anabolic steroid being assessed)and was used to assess systemic and systemic hormonal responses to androgens (Table 1). The rats were divided into three groups: oral androgens, oral anabolic androgens, or vehicle control rats. All other conditions were the same as those described above.TABLE 1Table 1. Effects of androgens on hormonal response to androgenic and anabolic steroids in rats subjected to three different doses.Preliminary analyses indicated that, at high doses, oral androgens significantly increased the total testosterone and androstenedione levels in the serum of males.At low-dose, the combination of oral anabolic agents potentiated the anabolic effects of androgens in females, whereas the combination of oral androgens potentiated the androgenic effects of androgens in males.There was very high inter-individual variability in the response to oral androgen administration in rats, although the total testosterone responses remained similar at higher doses. With the possible exception of the response to oral androgens, there was no significant interaction between, male sex and anabolic steroid. The total and total androstenedione responses to oral androgens were greater (Figure 1) in females than in males after high-dose administration. However, the anabolic effects of androgens were greater in females than in males after low-dose administration.All the other steroids had opposite-sex effects (i.e., the total testosterone response was greater in males than in females). However, the combined effects of androgens and anabolic steroids were greater at high-dose administration than in low-dose administration.These findings are of note because a higher dose of anabolic steroids has been reported to increase androgenic and androgenic steroid receptor expression and activity (25,26-29).The effect of anabolic androgenic steroids on the expression of estrogen receptors in the brain has been evaluated with the use of magnetic resonance imaging (MRI) and PET techniques. The findings have been presented previously (30,31).Testicular growth, a common indication with steroid use, has not been associated with the changes in androgen concentrations seen.The steroid response to androgenic and anabolic steroids in males did not differ significantly in the same groups of rats in several biochemical measures of estrus secretion. Moreover, no inter-individual differences were found in any of these measures, suggesting that steroid hormones did not have a significant effect on the estrus/leprous cycle in this group ofSimilar articles: